anti- 14-3-3 antibody

anti- 14-3-3 antibody

Product Name

14-3-3 antibody

Size

100µg

Form

liquid

Purification

Protein A+G purification

Purity

≥95% as determined by SDS-PAGE

Host

Mouse

Clonality

monoclonal

Isotype

IgG1

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

14-3-3 theta(also known as YWHAQ, or 14-3-3 tau) is a member of 14-3-3 proteins which were the first phosphoserine/phosphothreonine-binding proteins to be discovered. 14-3-3 family members interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms(gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo-and hetero-dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis. This antibody was raised against full-length 14-3-3 theta.

IMMUNOGEN INFORMATION

Immunogen

tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide

Synonyms

14 3 3, 14 3 3 protein T cell, 14 3 3 protein tau, 14 3 3 protein theta, 1C5, HS1, Protein HS1, YWHAQ

Observed MW

28 kDa

APPLICATION

Tested Application

ELISA, WB, IHC, IF

Recommended Dilution

WB: 1:1000-1:10000; IHC: 1:20-1:200; IF: 1:20-1:200

UNIPROT INFORMATION

UniProt ID

P27348

IMAGES

Immunohistochemistry of paraffin-embedded rat brain tissue slide using FNab00002(14-3-3 Antibody) at dilution of 1:50

Immunofluorescent analysis of HepG2 cells using FNab00002(14-3-3 Antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L)

human brain tissue were subjected to SDS PAGE followed by western blot with FNab00002(14-3-3 antibody) at dilution of 1:5000