anti- 4EBP1 antibody

anti- 4EBP1 antibody

Product Name

4EBP1 antibody

Size

100µg

Form

liquid

Purification

Protein A+G purification

Purity

≥95% as determined by SDS-PAGE

Host

Mouse

Clonality

monoclonal

Isotype

IgG2b

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Eukaryotic translation initiation factor 4E-binding protein 1(4EBP1), is a member of 4EBPs family, which regulate the translation of a subset of mRNA by competing with eIF4G for binding to eIF4E, thus preventing the assembly of the eIF4F complex. The eIF4F facilitates the recruitment of other translation initiation factors to form the complex and then initiates cap-dependent translation.4EBP1 also mediates the regulation of protein translation by growth factors, hormones and other stimuli that signal through the MAP kinase and mTORC1 pathways. There are three forms of 4EBP1, alpha, beta and gamma.

IMMUNOGEN INFORMATION

Immunogen

eukaryotic translation initiation factor 4E binding protein 1

Synonyms

4E BP1, 4EBP1, BP 1, eIF4E binding protein 1, EIF4EBP1, PHAS I

Observed MW

12 kDa

APPLICATION

Tested Application

ELISA, WB, IF

Recommended Dilution

WB: 1:500-1:2000; IF: 1:20-1:200

UNIPROT INFORMATION

UniProt ID

Q13541

IMAGES

Immunofluorescent analysis of Hela cells using FNab00006 ( 4EBP1 Antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L)

K-562 cells were subjected to SDS PAGE followed by western blot with FNab00006(4EBP1 antibody) at dilution of 1:2000